Structure and Function Analysis of Cultivated <i>Meconopsis integrifolia</i> Soil Microbial Community Based on High-Throughput Sequencing and Culturability

(1) Background: The structure, function, and community interactions of soil microbial communities of cultivated Meconopsis integrifolia were characterized by studying this alpine flower and traditional endangered Tibetan medicine. (2) Methods: Soil bacteria and fungi were studied based on high-throughput sequencing technology. Bacteria were isolated using culturomics and functionally identified as IAA-producing, organic phosphorus-dissolving, inorganic phosphorus-dissolving, and iron-producing carriers. (3) Results: The dominant bacterial phyla were found to be Proteobacteria and Acidobacteria, and unclassified_Rhizobiales was the most abundant genus. Ascomycota and Mortierellomycota were the dominant fungal phyla. The bacteria were mainly carbon and nitrogen metabolizers, and the fungi were predominantly Saprotroph—Symbiotroph. The identified network was completely dominated by positive correlations, but the fungi were more complex than the bacteria, and the bacterial keystones were unclassified_Caulobacteraceae and Pedobacter. Most of the keystones of fungi belonged to the phyla Ascomycetes and Basidiomycota. The highest number of different species of culturable bacteria belonged to the genus Streptomyces, with three strains producing IAA, 12 strains solubilizing organic phosphorus, one strain solubilizing inorganic phosphorus, and nine strains producing iron carriers. (4) Conclusions: At the cost of reduced ecological stability, microbial communities increase cooperation toward promoting overall metabolic efficiency and enabling their survival in the extreme environment of the Tibetan Plateau. These pioneering results have value for the protection of endangered Meconopsis integrifolia under global warming and the sustainable utilization of its medicinal value

A Comparison of Research Methods to Determine the Sustainability of Mineral Resources in Henan Province Based on Cloud Analysis

In order to improve the fuzzy comprehensive evaluation model of mineral resource sustainability and enhance its scientific and objective nature, in this paper, a cloud model-based risk assessment method is introduced to determine the sustainability of mineral resources in a comprehensive comparison, while using a combination of subjective and objective weighting method combining improved hierarchical analysis and the entropy weighting method. Compared with the previous single-assignment evaluation method, the method used in this paper has the advantages of more reasonable determination of weights, more accurate results and better visualization. On this basis, the combined weight method, cloud model method and hierarchical fuzzy evaluation method are organically combined to conduct a comprehensive evaluation of the sustainability of mineral resources in Henan Province. The case analysis shows that the comprehensive evaluation results of the sustainability of mineral resources obtained according to the method are scientifically reasonable and have important reference value and promotion significance for quantitative research in related fields

Dynamic Tensile Mechanical Properties of Outburst Coal Considering Bedding Effect and Evolution Characteristics of Strain Energy Density

The evolution of strain energy density of outburst-prone coal is of great significance for analyzing the characteristics of energy accumulation and release in coal and rock masses. The dynamic mechanical properties of coal samples were tested by using the split Hopkinson pressure bar (SHPB) technique. Dynamic tensile mechanical properties, layered effect and density evolution characteristics of strain energy for coal were studied. The dynamic failure and crack propagation process of the specimen were recorded with a high-speed camera. In addition, a digital image correlation (DIC) method was used to analyze the evolution characteristics of the strain field during the deformation process of the specimen. The distribution characteristics of the particle fragments were statistically analyzed. The results show that the bedding orientation of the coal has a significant effect on its deformation and damage features. The presence of weak planes, microcracks and laminae causes its shear damage zone to behave more complex. If the crack plane coincides with the high shear stress plane, the developed shear cracks extend along the weak laminae and the shear damage zones in BD specimens are not symmetrically distributed. When the laminated surface of the coal sample is at a certain angle with the impact loading direction, the damage mode is coupled with tensile and shear damage. The percentage mass distribution of particles and fines increases with increasing bedding orientation. The effect of water on the dynamic damage of coal samples is significant. Based on the principle of pressure expansion of wing-shaped cracks, the formula for calculating the dynamic strength of water-saturated coal samples under dynamic loading was derived

Individual Trajectories of Health Status During the First Year of Discharge From Hospitalization for Heart Failure and Their Associations With Death in the Following Years

Background Improving health status is one of the major goals in the management of heart failure (HF). However, little is known about the long‐term individual trajectories of health status in patients with acute HF after discharge. Methods and Results We enrolled 2328 patients hospitalized for HF from 51 hospitals prospectively and measured their health status via the Kansas City Cardiomyopathy Questionnaire–12 at admission and 1, 6, and 12 months after discharge, respectively. The median age of the patients included was 66 years, and 63.3% were men. Six patterns of Kansas City Cardiomyopathy Questionnaire–12 trajectories were identified by a latent class trajectory model: persistently good (34.0%), rapidly improving (35.5%), slowly improving (10.4%), moderately regressing (7.4%), severely regressing (7.5%), and persistently poor (5.3%). Advanced age, decompensated chronic HF, HF with mildly reduced ejection fraction, HF with preserved ejection fraction, depression symptoms, cognitive impairment, and each additional HF rehospitalization within 1 year of discharge were associated with unfavorable health status (moderately regressing, severely regressing, and persistently poor) (P<0.05). Compared with the pattern of persistently good, slowly improving (hazard ratio [HR], 1.50 [95% CI, 1.06–2.12]), moderately regressing (HR, 1.92 [1.43–2.58]), severely regressing (HR, 2.26 [1.54–3.31]), and persistently poor (HR, 2.34 [1.55–3.53]) were associated with increased risks of all‐cause death. Conclusions One‐fifth of 1‐year survivors after hospitalization for HF experienced unfavorable health status trajectories and had a substantially increased risk of death during the following years. Our findings help inform the understanding of disease progression from a patient perception perspective and its relationship with long‐term survival. Registration URL: https://www.clinicaltrials.gov; unique identifier: NCT02878811